To study the influence of temperature on the measurement of wind tunnel strain-gage balance, the calibration technology under the action of thermo-mechanical coupling is taken as the focus of the research. The thermo-mechanical coupling loading matrix is established through the response surface experimental design method. The thermo-mechanical coupling loading is implemented on a six components wind tunnel strain-gage balance based on the six-degree of freedom calibration system and the constructed temperature environment. The balance formula including temperature, force and moment parameters is established by the multivariate regression method. Finally, the verification load is used to test the accuracy of the balance formula. Results show that the comprehensive loading error of each component of the balance is better than 0. 3% under the action of temperature, force and moment load. The balance formula can accurately characterize the comprehensive performance of the balance under the action of thermo-mechanical coupling. Results show that the calibration technology of wind tunnel strain-gage balance under the action of thermo-mechanical coupling can be used to evaluate and correct the influence of temperature on balance measurement.