The mechanical wear of adjustment mechanism in the conventional gene biochip imaging system caused by frequent adjustment of gene chip pose is a key problem. To address this issue, a gene biochip imaging scanner based on magnetic levitation is designed. According to genetic biochip imaging scanner work principle and structure characteristics of magnetic suspension technology, a system is established, which consists of electromagnetic parameters and the imaging resolution differential array. Through the theory of optimization, the structure parameters are determined. The finite element analysis is used to analyze the system structure of electromagnetic heat coupling, optimize analysis results and set up the experimental test equipment. The device is used to calibrate the parameters of the gene biochip imaging scanner, verify the magnetic levitation system structure design results of the scanner, and compare the experimental test data with the imported PCR instrument. Results show that when the number of turns of electromagnetic structure of the designed magnetic levitation genetic biological imaging scanner is 340 N, the effective electromagnetic area is 180 mm 2 , and the accuracy error of magnetic levitation system is less than 0. 15 mm. They are basically consistent with the simulation data. Compared with the data of T790M mutation detection by bio-RAD digital PCR system in the United States, CV is less than 5% . The precision of the designed gene-biochip imaging scanner based on magnetic levitation can meet the requirements of gene-biochip imaging detection, provide core technical support for improving the service life of the adjustment device, and play an important role in improving the gene-diagnosis technology of clinical tumor screening.