The collection and control of micro-fluid are key technologies in the field of microfluidics. The introduction control of microfluid requires not only the precise control of velocity, time and other parameters, but also the avoidance of cross contamination of fluids in multiple detection areas. In this article, a microfluidic liquid-introduction-control chip based on the bursting valves is designed. Firstly, the mechanism of the bursting valve is analyzed and the microfluidic chip is fabricated by the MEMS process. Then, the hydrophilicity of PDMS that affects the characteristics of the bursting valves is tracked. The liquid introduction characteristics of the microfluidic chip are observed at both macro and micro levels. Furthermore, the theoretical calculation and experimental measurement of the bursting pressure of the bursting valves are carried out, and the measured bursting pressure values of the three types of bursting valves are 90, 690 and 2 440 Pa, respectively. Finally, the blasting characteristics of the bursting valves under different injection rates are tested. Experimental results show that, through the reasonable design of the bursting valves, the introduction control of micro-fluid can be effectively controlled. The liquid can flow into the detection area in a predetermined order and time, and the cross contamination of fluids can be avoided effectively.