The TAS2Rs in extra-oral tissues may become a potential therapeutic target in the treatment of related diseases. In this study, cells expressing of TAS2Rs are used as sensitive elements. According to their physiological characteristics and coupling with different sensors, the heterotopic expression of taste receptors and their application in personalized drug screening are explored. The personalized drug screening platform for different disease models is established. Firstly, based on the electric cell-substrate impedance sensing (ECIS) and the endogenous T2R38 receptor expressing Caco2 cells, a drug screening platform for isothiocyanates is developed. The EC50 of phenylthiourea is calculated as 157. 6 μM. Secondly, combined with the microelectrode array (MEA) detection system, the inhibitory effects of diphenidol ( 5 ~ 160 μM) and salicin ( 0. 001 ~ 100 μM) on the contraction of cardiomyocytes are investigated. Furthermore, based on three-dimensional airway smooth muscle cells (ASMCs) array and the gel imaging system, the diastolic effect of tangeretin (20 μM) on airway smooth muscle cells is explored.