Temperature is the important function that affects the normal function of human body. Blood temperature change monitoring is particularly important for the assessment of human health. Based on the proposed extended HANAI theory of the dense dispersion system, the influence of temperature on the impedance characteristics of blood has been quantitatively evaluated by bioelectrical impedance spectroscopy measurement, which provides a feasible quantitative characterization basis for blood temperature and related blood disease monitoring. Experimental results show that, under the condition that the temperature T increases, the impedance Z ∗ decreases continuously. It founds that the resistance Rc and reactance Xc have great linear relationship with the temperature T, which could be described as Rc = -0. 3T+24. 14 and Xc = 0. 08T-5. 78, respectively. The tranditional HANAI equation for dense solution is extended. The numerical analysis and simulation results show great agreement with experimental results which confirm the fact that the conductivities σp of plasma and the conductivities σc of cytoplasm are responsible for the influence of temperature on blood impedance characteristics. This study indicates a potential non-invasive temperature measurement method for blood or other solutions, which has certain scientific research and application value.