Abstract:As an important step to calibrate the static working radius of a 10-6 scale precision centrifuge, the accuracy of the rotation axis determination will directly affect the measurement uncertainty of the output acceleration. According to the definition, the rotation axis used as a virtualdatum cannot bedirectly obtained withmeasurement. Aiming attheabove problems,a measurement method fortherotation axisof precisioncentrifuge basedonparameterestimation isproposed usingGaussian filtering, eccentricity andtiltparameter estimation, and automatic adjustment. The method can make the geometric center line of standard cylinder rod coincide with the rotation axis, and achieves the purpose of datum conversion. Roundness meter and emulation experiments were used to verify the correctness and effectiveness of the filtering algorithm and the parameter estimation. A rotation axis measurement unit was built using precision turntable, standard cylinder rod, spectral confocal sensor and six axis parallel positioning motion platform. The test results demonstrate that the eccentricity between the standard cylinder rod and the rotation axis can be adjusted to 02 μm, and the tilt can also be adjusted to 0000 2°.