Abstract:Fluorescencebased sensing technology belongs to the multidiscipline fusion of sensors and transducers. Especially, research on physical quantity is in the startup stage. The principles and methods of fluorescence temperature/force/displacement/humiditysensing techniques are introducted. And some examples are illustrated, including our researches. Fluorescence lifetime, fluorescence intensity quenching, fluorescence intensity ratio, fluorescence spectral shift, and fluorescence colormetric analysis processes are discussed. The typical sensing properties are compared with other techniques. A particular note is that fluorescence lifetime is the other side of fluorescence intensity quenching. Data processing is introduced, which focuses on how to improve the sensing precision with a novel spectral characteristic, named as “emission band barycenter”. At the end, the future development and applications are provided.