With regard to the traditional laser microdissection using the relative motion between the single laser focus and the workpiece, there are limitations in forming the machining trajectory, such as the low cutting efficiency and the difficulty in closing the cutting trajectory. To address these issues, a laser microdissection method based on digital micromirror device without mechanical movement is proposed. By adjusting the digital micromirror array, the amplitude of the laser beam can be adjusted, and the arbitrary light field intensity distribution can be obtained in the sample area with the optical path design. In this way, the parallel cutting of plane projection is realized with almost the same intensity. Meanwhile, the process of key parameter design, simulation, and system construction is described. Specifically, the polyester film is first cut with various patterns such as straight line, hollow ring and hollow rectangle. Then, the frozen section of cell tissue is cut. Experiments show that, by one projection, designable patterned structures can be cut in parallel with accurate target graphics and high cutting efficiency. Under the 20× objective lens, the minimum cutting line width reaches submicron, and the minimum ring diameter is smaller than the single cell diameter. This method can provide new ideas and new directions for improving the efficiency and accuracy of the laser microdissection system.