The filter wheel-based spectral imaging system is widely used in spectral imaging, its spatial resolution is high, however, its spectral resolution is low. Aiming at this problem, a high spectral resolution imaging based on filter wheel dual camera system is introduced in this paper, meanwhile, a multi-spectral calculation and reconstruction method based on interpolation compensation is designed to achieve high spectral resolution and high spatial resolution imaging of the system. Firstly, the filter wheel dual camera imaging system is used to acquire the multi-spectral images and RGB images, and then the discrete spectral response curves are obtained from the multi-spectral images. Finally, according to the mapping relationship between the RGB three channel data and the spectral high-dimensional data, and the theorem of conservation of energy, the interpolation compensation of the spectral response curve is performed and the high spectral resolution imaging is achieved. Experiment results show that the proposed method can efficiently achieve the imaging with a spectral resolution of 5 nm even higher while maintaining the spatial resolution. The root mean square error between the reconstruction result and the true value is 0. 017 1, the proposed method has high accuracy and robustness.