Isothermal titration calorimetry (ITC) is an important method for the thermodynamic measurement of solution reaction. Based on the quantitative evaluation of ITC basic error sources, an explicit ITC measurement model is formulated, which consists of both different thermal effects and main error components. By utilizing this ITC measurement model, BaCl 2 / 18-crown- 6, CaCl 2 / EDTA and 1-Propanol dilution are used to calibrate the calorimetric factor (f) and the effective volume of the reaction cell (Vcell) of the NanoITC (SV) calorimeter. Based on the concentration uncertainty of the titration solution, the basic error terms of the calorimeter and the uncertainty of the theoretical values of the reaction system, the calibration uncertainties can be calculated by the Monte Carlo method. Three groups of different calibration experiments are compared with each other. The results show that an 8. 1% deviation in the calorimetric factor and a 3. 4% deviation in the effective volume of the reaction cell. Supplementary materials are provided in terms of experimental data and Python source code.